Hieff qpcr sybr
Web7 de set. de 2024 · The other components including 2 × Hieff ® qPCR SYBR Green Master Mix 5 μL, standard plasmid DNA template (1.0 × 10 3 copies/μL) 1 μL, and nuclease-free water were added to make a total volume of 10 μL. The amplification reaction was performed in the LightCycler ... Web5 de abr. de 2024 · RT-qPCR was performed using Hieff® qPCR SYBR Green Master Mix (Low Rox Plus) (YEASEN) on an AB7500 machine. The following primers were used: AFP (forward primer: GGAAGTCTGCTTTGCTGAAGA, reverse primer: CACACCGAATGAAAGACTCGT); GAPDH (forward primer: …
Hieff qpcr sybr
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WebApplied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain … WebMaster mixes SYBR Green da Applied Biosystems foram projetadas para PCR em tempo real quantitativo que usam um conjunto de dois primers que flanqueam a região alvo. …
Web20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was ... The total volume of the reaction system was 20 μL, including Hieff® qPCR SYBR Green Master Mix, 10 μL, Forward primer, 0.4 μL, Reverse primer, 0.4 μL, … Web湖南医药学院第一附属医院关于其他互作相关试剂的网上超市采购项目合同履约验收公告
WebSYBR® Green Realtime PCR Master Mix 1 ml SYBR® Green Realtime PCR Master Mix 1 ml x 5 该组分是包含反应缓冲液、SYBR® GreenⅠ、dNTPs、Mg2+、Taq … WebSYBR Green master mixes are a fast and convenient means of carrying out quantitative PCR (qPCR) experiments. SYBR Green is a cyanine fluorophore that intercalates with and stains double-stranded DNA. ... 2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix …
Web4 de mai. de 2024 · The Hifair ® II 1st Strand cDNA Synthesis Kit (gDNA digester plus) and Hieff ® qPCR SYBR Green Master Mix (Low Rox Plus) from Yeasen Biotechnology Co., Ltd (Shanghai, China) were used for cDNA synthesis and qRT-PCR. The primer pairs for qPCR (Table S7) of the eight selected genes with annotation were designed by Oligo7.
WebThe 2×Hieff™ miRNA Universal qPCR SYBR Master Mix is a new generation of premix fluorescent quantitative PCR detection reagent specially developed for the quantitative detection of miRNA. It contains special ROX Passive Reference Dye. Suitable for most qPCR instruments, there is no need to adjust the concentration of ROX on different ... how many people get rheumatoid arthritisWebUpdated SYBR Green Master Mix formulation: PowerTrack SYBR Green Master Mix is our newest formulation designed to greatly simplify your work and deliver high sensitivity and … how can i send an efaxWeb6 de abr. de 2024 · The cDNA was then used for the RT-qPCR analysis using specific primers (Table S1) and Hieff® qPCR SYBR Green Master Mix (YE SEN, China). RT-qPCR was performed using the CFX96 Touch™ real-time PCR Detection System (BioRad, USA). β-actin was used as an internal reference gene. The qPCR program was as follows: 5 … how can i send an email to sbiWeb20 de ago. de 2024 · Then, Hieff® qPCR SYBR Green Master Mix (Low Rox Plus) (Yeasen, Shanghai, China) was adopted for qPCR, and qPCR was performed in an ABI-Viia7 instrument (ABI, America). More detailed methods were described in our previous research (Wang et al. 2024). how many people get scammed a dayWebAccumulating studies demonstrate that the expressions of hypoxia-inducible factor 1 (HIF-1), erythropoietin (EPO) and vascular endothelial growth factor (VEGF) depend on cellular … how many people get possessed a yearWeb11 de fev. de 2024 · Hieff qPCR SYBR ® Green Master Mix可精确分辨5倍，甚至低至2倍的模板量差异。而且在宽广的定量区域内表现良好。 3. 重复性高 图3. 以人293T细胞cDNA … how can i send an email as a text messageWebHieff ® qPCR SYBR Green Master Mix(Low Rox Plus)是2×实时定量PCR扩增的预混合溶液。 Mix中含有热启动Hieff® DNA Polymerase、SYBR Green I、dNTPs、Mg2+及Low … how can i send an email to dana perino